The final day was led by a Galileo filly from Barronstown Stud who realised 2,100,000 guineas, the top priced filly at the sale.
The filly is out of the G2 winner Quiet Lass and was knocked down to MV Magnier and George von Opel's Westerberg.
"She is a lovely filly and her full-brother Lancaster House is a good horse," said MV Magnier.
Lancaster House, a three-year-old of this year, is now the winner of three races, including the Listed Listowel Stakes.
Winx's Australian breeder John Camilleri sold a Kingman colt for 1.8 million guineas
Adrian and Philippa O'Brien's Hazelwood Bloodstock continued a remarkable sale when they offered the colt out of Onr Last Dance. He was purchased by MV Magnier.
"It is very special to be here and sell here," said Camilleri. "I have a small band of around 12 broodmares in Europe and they are in Ireland at Norelands and in the UK at Hazelwood.
"It is a mix of both European and southern hemisphere bloodlines.
"I sent up some mares from Australia. They were not in-foal on southern hemisphere time but I thought their bloodlines were good enough to come to the northern hemisphere.
"The stallions whom I would call 'elite' are currently in Europe - Dubawi, Kingman, Galileo and Frankel."
The underbidder on the quality colt was Godolphin's Anthony Stroud whilst others to get a bid in included Gold medal winning Olympic eventer Mark Todd.
Book 1 of the Tattersalls October Yearling Sale has been a memorable one for the O'Briens with their three sold lots realising 5,875,000 guineas in total.
A Frankel brother to the 2015 Epsom Derby winner Golden Horn sold for 3,100,000 guineas, the second highest price for a yearling colt in the world this year.
He lit up the ring at Tattersalls on the second day of Book 1 of the October Yearling Sale when selling to Godolphin from Harry McCalmont's Norelands Stud.
He is a son of the Dubai Destination mare Fleche D'Or.
It was a superb result for Norelands, the consignor having sold Lot 208 to MV Magnier for 1,300,000gns earlier today.
"I am not often lost for words, but I think I am now," smiled McCalmont. "This is easily the best price we have had in the sales ring.
"There were some serious people who liked him, however you never know what is going to happen until you get to the ring."
McCalmont bought the mare Fleche D'Or at the Tattersalls December Mares Sale in 2012 for 62,000 guineas after she had foaled the subsequent champion three-year-old colt Golden Horn.
"I like buying mares from such successful breeders as Anthony Oppenheimer," said McCalmont. "You know the mares in the pedigree will always get the best covers and go to the best trainers."
The 13-year-old Fleche D'Or had a Frankel filly foal this spring, who will be retained, and she is in-foal to Sea The Stars.
A Kingman colt out of the Montjeu mare Grace And Favour became the result of a lifetime for Nicolas Jones' Coln Valley Stud when selling to Godolphin for 2,300,000 guineas.
The son of Juddmonte Farms' sire sensation is out of a half-sister to five-time G1 winner Fame And Glory and to the dam of 1,000 Guineas winner Legatissimo.
The colt was knocked down to Anthony Stroud on behalf of Godolphin.
"Our previous best came last year when we sold a Showcasing colt out of Grace And Glory for 600,000 guineas," said Jones.
"We have only six Flat mares and one NH mare we like to keep the numbers to this size and maintain the quality. We sell the colts and keep the fillies."
Grace And Favour had a Pivotal filly foal this year and is in-foal to Sea The Moon.
Jones bought Grace And Favour's dam Gryada in 2006 for 180,000 guineas at the Tattersalls December Mares Sale via agent Amanda Skiffington.
At the conclusion of Book 1 of the 2019 Tattersalls October Yearling Sale, Tattersalls Chairman Edmond Mahony commented;
"There have been some spectacular highlights at Book 1 of the 2019 Tattersalls October Yearling Sale including two outstanding colts breaking the 3 million guineas mark.
In total we have had ten yearlings make 1 million guineas or more reflecting the extraordinary quality of the yearlings assembled for Book 1.
"After eight consecutive years of growth with turnover rising from 51 million guineas in 2011 to last year's massive 106 million guineas, the aggregate has fallen a little short this year, but the sale has still produced turnover in excess of 100 million guineas which is the second highest in the sale's history.
"As ever, we should acknowledge the fantastic support we have received from Sheikh Mohammed and Sheikh Hamdan Al Maktoum as well as the Coolmore team. They have all made a major contribution to the very top of the market which has been highly competitive from start to finish, but a new dimension has been added by a particularly strong contingent of American buyers.
"Their presence has been a growing feature in recent years, and we have worked consistently hard promoting the sale throughout America, so it has been genuinely rewarding to see so many new American buyers here this week.
"They have made a real impact and it looks as if around 50 yearlings will be heading across the Atlantic."