Community TAB will match funds raised on the day for the Saving Animals From Euthanasia (SAFE) rescue centre in Karratha, up to $2000.
The race club will kick off the fundraising by donating 50 cents from every general admission ticket purchased and will have donation tins throughout the course during the day.
Nor West Jockey Club Secretary Leanne Mott welcomed the alliance between the race club and Community TAB.
"Community TAB does some amazing work in communities and, as a regional race club, we really appreciate the support the initiative provides. It means a lot that we can be involved," Leanne said.
"As one of our biggest race days of the year, we are expecting record crowds at the TABtouch Roebourne Cup day and it is a fantastic opportunity for people to dig deep.
"SAFE does exactly what the name says, Sue Hadley and her team of volunteers take in animals from all walks of life and find them forever homes. The money raised will assist with veterinary and rehoming expenses, as well as provide support and supplies for volunteer foster carers."
Racing and Wagering Western Australia Chair Jeff Ovens said the positive effects of the Community TAB program have been extensive for regional WA, with 48 of WA's race clubs based in regional communities.
"RWWA recognises that race clubs across WA play an important role in their communities, contributing both economically and socially, with partnerships like these an example of the strong connection race clubs have within the communities in which they operate," Jeff said.
"Community TAB is proud to play our part in giving back to the community, and our donation to SAFE is just one of many community organisations supported as part of the program."
The Community TAB dollar matching program began in 2015 and has since raised more than $700,000 for not-for-profit organisations around WA through partnerships with local race clubs.