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Swim star Ariarne Titmus' quest for a second gold of the Tokyo Olympics comes as cyclist Rohan Dennis pedals for a medal on Wednesday.

For Ariarne Titmus, this sequel may better the original.

Titmus meets American foe Katie Ledecky for the 200m freestyle gold medal as Australia enter Wednesday's competition with three gold, one silver and five bronze medals.

And much of the Tokyo Olympics will pause for the reprise of their heroic 400m freestyle final, when the Australian pipped the American great.

"I enjoy racing Katie - it's exciting for everyone and myself," Titmus said after her 200m semi-final.

"But the 200 is not just Katie and I. It's a very strong field ... it's anyone's race."

Titmus' medal race headlines action at the pool on a day when cycling star Rohan Dennis rides for Olympic redemption in the road time trial.

Dennis was well on the way to the podium in the same event at the Rio Games when a handlebar extension snapped, meaning a bike change that relegated him to fifth.

He bypassed last weekend's road race to focus on the time trial and is one of the medal favourites, while Grace Brown is a contender in the women's event.

The women's four are big medal chances in rowing after posting the fastest qualifying time.

The men's fours will try to dethrone Great Britain, who have won every gold medal in the event since Sydney.

After they row for gold in the fours on Wednesday, Jessica Morrison and Annabelle McIntyre will also back up for the pairs semi-finals.

The day after missing out on a gold medal in the K1, Jess Fox will back up in the opening two runs of the C1 in canoe slalom.

It's a busy day for tennis star Ash Barty - after she and John Peers play Argentina in the opening round of the mixed doubles, the world No.1 and Storm Sanders will take on the Czech Republic in the women's doubles quarter-finals.

Sam Stosur and Ellen Perez have a doubles quarterfinal against Switzerland.

The Boomers take on Italy in basketball and the two hockey sides are also in action, with Kookaburras playing New Zealand and the Hockeyroos going up against Japan.

The Olyroos play Egypt in men's soccer.

James Willett, Thomas Grice, Laetisha Scanlan and Penny Smith have qualification for the trap event in shooting.

Boxer Skye Nicolson is into the featherweight quarter-finals against Karriss Artingstall from Great Britain, as middleweight Caitlin Parker and light heavyweight Paulo Aokuso are in their preliminaries.